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. 2019 Nov 8;11(11):587. doi: 10.3390/pharmaceutics11110587

Figure 4.

Figure 4

Differences in the fold-change of gene expression between (a) BMEC and (b) bEnd.3 cells grown on a polyester membrane insert filter compared to cells grown on plastic cell culture flask. Relative gene expression was calculated using the 2−ΔΔCt method, with HPRT as the housekeeping gene and cells grown on the plastic cell culture flask as the calibrator. Data were analyzed using an unpaired t test with Welch’s correction (ns: p ≥ 0.0332, * p < 0.0332, ** p < 0.0021, *** p < 0.0002, **** p < 0.0001). The bars indicate the average mRNA fold change ± SD (n = 3).