Table 2.
Summary of mass spectrometry conditions.
| HPLC | Agilent 1100 Series | |||
| MS/MS | MDS Sciex 4000 Qtrap | |||
| Software | Analyst® (v1.6.2) | |||
| Ionisation source, mode | Turbo electrospray, positive ionisation | |||
| Scan mode | Multiple reaction monitoring (MRM) | |||
| Analyte parameters | Compounds | DP (V) | MRM | CE (eV) |
| Verapamil | 110 | 455.3 > 165.0 | 60 | |
| Midazolam | 90 | 326.2 > 291.1 | 42 | |
| Chlorpromazine | 65 | 319.2 > 86.0 | 28 | |
| Caffeine | 90 | 181.1 > 124.2 | 28 | |
| Atenolol | 41 | 267.1 > 145.0 | 45 | |
| Theophilline | 70 | 194.1 > 138.2 | 27 | |
| Tenoxicam | 71 | 337.3 > 121.0 | 33 | |
| Metochlopramide (ISTD) | 70 | 300.1 > 184.3 | 44 | |
| Source parameters | Gas temp (°C) | 550 | ||
| Gas flow (L/min) | 50 | |||
| Curtain gaz (psi) | 25 | |||
| Capillary (V) | 5500 | |||
| Mobile phase | Composition | A: 0.1% FA+ H2O | ||
| B: 0.1% FA + ACN | ||||
| Gradient | 2 to 98% B in 3.5 min | |||
| Flow rate | 0.75 mL·min−1 | |||
| Column temperature | 45 °C | |||
| Injection volume | 4 µL | |||
| Injection temperature | 5 °C | |||
| Column | YMC-Pack ODS-AQ, (50 × 3.0 mm, 5 µm) | |||