Table 3.

Primers sequences and parameters used in this study for the analysis of the mRNA expression of main transporters and tight junction proteins.

Target Protein/Abbreviation/Gene Symbol	Function	Reference Sequence	Forward/Reverse Primers
P-glycoprotein/Pgp/Abcb1a	Efflux transporter	NM_011076.2	gggcatttacttcaaacttgtca/tttacaagcttcatttcctaattcaa
Breast cancer resistance protein/BCRP/Abcg2	Efflux transporter	NM_011920.3	aggtctggaaaaagtagcagattc/ctccatccctatgcttgtcc
Glucose transporter-1/GLUT-1/Slc2a1	Uptake transporter	NM_011400.3	gtatcctgttgcccttctgc/tcgaagcttcttcagcacac
L-type amino acid transporter/LAT-1/Slc7a5	Uptake transporter	NM_011404.3	tcagcttcttcaactggctgt/ggagggccagattcacct
Zonula occludens-1/ZO-1/Tjp1	Tight junction	NM_009386.2, NM_001163574	cgcggagagagacaagatgt/gaagcgtcactgtgtgctgt
Occludin/OCL/Ocln	Tight junction	NM_008756.2	gtccgtgaggccttttga/ggtgcataatgattgggtttg
Claudin-5/CL-5/Cldn5	Tight junction	NM_013805.4	ttaaggcacgggtagcactc/atgttggcgaaccagcag
Hypoxanthine-guanine Phosphoribosyltransferase/HPRT/Hprt	Endogenous protein	NM_013556.2	tcctcctcagaccgctttt/cctggttcatcatcgctaatc