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. 2019 Nov 5;7(11):530. doi: 10.3390/microorganisms7110530

Table 2.

Sequences of primers used for intestinal microbiota in mice feces.

Gene Name Sequence
Firmicutes Forward: 5′-GGAGYATGTGGTTTAATTCGAAGCA-3′
Reverse: 5′-AGCTGACGACAACCARGCAC-3′
Bacteroides Forward: 5′-GGARCATGTGGTTTAATTCGATGAT-3′
Reverse: 5′-AGCTGACGACAACCATGCAG-3′
Actinobacteria Forward: 5′-TACGGCCGCAAGGCTA-3′
Reverse: 5′-TARTCCCCACCTTCCTCCG-3′
Proteobacteria Forward: 5′–CATGACGTTACCCGCAGAAGAA–3′
Reverse: 5′–CTCTACGAGACTCAAGCTTGC–3′
Bifidobacterium Forward: 5′-TCGCGTC(C/T)GGTGTGAAAG-3′
Reverse: 5′-CCACATCCAGC(A/G)TCCAC-3′
Lactobacillus Forward: 5′-AGCAGTAGGGAATCTTCCA-3′
Reverse: 5′-CACCGCTACACATGGAG-3′
Enterobacteriaceae Forward: 5′-CATTGACGTTACCCGCAGAAGAAGC-3′
Reverse: 5′-CTCTACGAGACTCAAGCTTGC-3′
Universal primer Forward: 5′-ACTCCTACGGGAGGCAGCAG-3′
Reverse: 5′-ATTACCGCGGCTGCTGG-3′