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. 2019 Nov 8;9(11):718. doi: 10.3390/biom9110718

Figure 3.

Figure 3

Depletion of LncRNA RAB5IF induces apoptosis in HCCs. (a) Effect of LncRNA RAB5IF depletion on cell cycle distribution in HepG2 and Hep3B cells by Fluorescence-activated cell sorting (FACS). Data represent means ± SD by three independent experiments. ** p < 0.01 and *** p < 0.001 vs. siRNA control. (b) After transient transfection of HepG2 and Hep3B cells with LncRNA RAB5IF siRNA, Annexin- Propidium Iodide (PI)staining assays was performed. The cells were stained using Fluorescein isothiocyanate (FITC)-Annexin V/PI dye and early and late apoptotic portions were detected by flow cytometry. (c) Effect of LncRNA RAB5IF depletion on apoptosis related genes in HepG2 and Hep3B cells by Western blotting. Cell lysates were prepared and subjected to Western blotting for PARP, Caspase3 and Bcl-2. Graphs represent relative level of protein expression /β-actin. Data represent means ± SD by two independent experiments. * p < 0.05, ** p < 0.01 and *** p < 0.001 vs. siRNA control.