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. 2019 Nov 8;9(11):718. doi: 10.3390/biom9110718

Figure 4.

Figure 4

LncRNA RAB5IF regulates c-Myc and LGR5 in HCCs. (a) LGR5 RNA expression in various human cancer cell lines by qRT-PCR. Data represent means ± SD by two independent experiments. ** p < 0.01 and *** p < 0.001 vs. LGR5 level in MCF-7 cells. (b) Effect of LncRNA RAB5IF depletion on LGR5 pathway in HepG2 and Hep3B cells by Western blotting. Cell lysates were prepared and subjected to Western blotting for LGR5, β-catenin and c-Myc. Graphs represent relative level of protein expression /β-actin. Data represent means ± SD by two independent experiments. ** p < 0.01 and *** p < 0.001 vs. siRNA control. (c) HepG2 and Hep3B cells were transfected with LncRNA RAB5IF siRNA for 48 h and then transfected with Lv-LncRNA RAB5IF and Lv-con viruses for 24 h. Cell lysates were prepared and subjected to Western blotting for LGR5, β-catenin, c-Myc and Pro-PARP.