Figure 6.

Effect of irs knockdown on total lifespan for the high pollen hoarding strain by host colony. In the high strain, irs knockdown (hIRS) resulted in decreased lifespan relative to the GFP control (hGFP) in both colonies (a) colony 1: Kaplan–Meier: χ² = 14.9, p = 0.0006, n = 411, Cox–Mantel: p = 0.0052, (b) colony 2: Kaplan–Meier: χ² = 28.3, p < 0.0001, n = 638, Cox–Mantel: p = 0.001. There was no effect of handling on total lifespan in the high strain (Cox–Mantel: hGFP vs. hNoI, colony 1: p = 0.482, colony 2: p = 0.52).