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Effect of irs knockdown on total lifespan for the low pollen hoarding strain by host colony. In the low strain, the effect of irs knockdown (lIRS) relative to GFP-injected control (lGFP) on total lifespan was not significant in (a) colony 1 (Kaplan–Meier: χ2 = 5.9, p = 0.051, n = 446, Cox–Mantel: p = 0.054). (b) In colony 2 irs knockdown resulted in decreased total lifespan (Kaplan–Meier: χ2 = 10.6, p = 0.0051, n = 476, Cox–Mantel: p = 0.002).
