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. 2019 Nov 8;7(11):542. doi: 10.3390/microorganisms7110542

Table 1.

Total average value of protein content (mol%) of the proteins related to apoptosis and autolysis identified in S. cerevisiae P29 and G1 under pressure (PC) and non-pressure (NPC) conditions, and in each sampling time: at the middle of the secondary fermentation (T1) and 1 month after it (T2). Total proteome identified so far in S. cerevisiae is provided. A–e Different letters in a row represent significant differences of protein content in each condition at 0.05 level according to Fisher’s least significant difference procedure. n.f. not found.

Condition PC T1 NPC T1 PC T2 NPC T2
Yeast Strain P29 G1 P29 G1 P29 G1 P29 G1
mol% ± SD mol% ± SD mol% ± SD mol% ± SD mol% ± SD mol% ± SD mol% ± SD mol% ± SD
Total Apoptosis 1.7b ± 0.2 2.5c ± 0.3 1.2a ± 0.2 2.7c ± 0.2 3.2d ± 0.3 4.5e ± 0.2 3.4d ± 0.4 3.5d ± 0.3
Total Autolysis 3.3a ± 0.4 3.7a ± 0.7 5bc ± 1 3.3a ± 0.8 3.3a ± 0.5 4.9bc ± 0.3 3.7ab ± 0.4 5.9c ± 0.5
Glucanases 0.73b ± 0.08 0.51a ± 0.04 0.7b ± 0.2 0.6ab ± 0.1 1.2c ± 0.2 2.4e ± 0.1 1.7d ± 0.2 2.18e ± 0.09
Mannosidases n.f.a n.f.a 0.13c ± 0.05 0.05b ± 0.02 0.022b ± 0.001 n.f.a n.f.a 0.104c ± 0.001
Non-vacuolar proteases 1.5c ± 0.3 1.6c ± 0.4 2.94d ± 0.8 1.5c ± 0.4 0.8b ± 0.2 n.f.a 0.6b ± 0.1 1.4c ± 0.2
Vacuolar proteases 0.9a ± 0.1 1.3c ± 0.2 0.9a ± 0.2 1.0ab ± 0.2 0.9a ± 0.1 2.6d ± 0.2 1.2bc ± 0.1 2.3d ± 0.2
Nucleases 0.18c ± 0.01 0.3de ± 0.1 0.25cd ± 0.04 0.18c ± 0.1 0.43e ± 0.02 n.f.a 0.29d ± 0.02 0.06b ± 0.02