Table 1.
Total average value of protein content (mol%) of the proteins related to apoptosis and autolysis identified in S. cerevisiae P29 and G1 under pressure (PC) and non-pressure (NPC) conditions, and in each sampling time: at the middle of the secondary fermentation (T1) and 1 month after it (T2). Total proteome identified so far in S. cerevisiae is provided. A–e Different letters in a row represent significant differences of protein content in each condition at 0.05 level according to Fisher’s least significant difference procedure. n.f. not found.
| Condition | PC T1 | NPC T1 | PC T2 | NPC T2 | ||||
|---|---|---|---|---|---|---|---|---|
| Yeast Strain | P29 | G1 | P29 | G1 | P29 | G1 | P29 | G1 |
| mol% ± SD | mol% ± SD | mol% ± SD | mol% ± SD | mol% ± SD | mol% ± SD | mol% ± SD | mol% ± SD | |
| Total Apoptosis | 1.7b ± 0.2 | 2.5c ± 0.3 | 1.2a ± 0.2 | 2.7c ± 0.2 | 3.2d ± 0.3 | 4.5e ± 0.2 | 3.4d ± 0.4 | 3.5d ± 0.3 |
| Total Autolysis | 3.3a ± 0.4 | 3.7a ± 0.7 | 5bc ± 1 | 3.3a ± 0.8 | 3.3a ± 0.5 | 4.9bc ± 0.3 | 3.7ab ± 0.4 | 5.9c ± 0.5 |
| Glucanases | 0.73b ± 0.08 | 0.51a ± 0.04 | 0.7b ± 0.2 | 0.6ab ± 0.1 | 1.2c ± 0.2 | 2.4e ± 0.1 | 1.7d ± 0.2 | 2.18e ± 0.09 |
| Mannosidases | n.f.a | n.f.a | 0.13c ± 0.05 | 0.05b ± 0.02 | 0.022b ± 0.001 | n.f.a | n.f.a | 0.104c ± 0.001 |
| Non-vacuolar proteases | 1.5c ± 0.3 | 1.6c ± 0.4 | 2.94d ± 0.8 | 1.5c ± 0.4 | 0.8b ± 0.2 | n.f.a | 0.6b ± 0.1 | 1.4c ± 0.2 |
| Vacuolar proteases | 0.9a ± 0.1 | 1.3c ± 0.2 | 0.9a ± 0.2 | 1.0ab ± 0.2 | 0.9a ± 0.1 | 2.6d ± 0.2 | 1.2bc ± 0.1 | 2.3d ± 0.2 |
| Nucleases | 0.18c ± 0.01 | 0.3de ± 0.1 | 0.25cd ± 0.04 | 0.18c ± 0.1 | 0.43e ± 0.02 | n.f.a | 0.29d ± 0.02 | 0.06b ± 0.02 |