Figure 5.

The inhibitory effect of polyphenols on the fibrillation of different FapC mutants. 30 µM of purified mutants of FapC-UK4 was incubated with 100 µM polyphenols in Tris buffer (pH 7.5) with shaking at 37 °C. (A) Absolute lag time (t_N) in the absence of polyphenols, (B) normalized maximum ThT emission of different mutants in the presence of inhibitors, (C) relative half time (t_N/t_N,control); (D) SDS-PAGE of different mutants incubated with EGCG; (E) quantification of monomer, dimer, and oligomer bands from panel e using Image J.