Table 1.

Experiments of classical absolute conditioning conducted on two consecutive days to assess the effect of scented-US reward and previous olfactory experience on olfactory learning in the stingless bee Melipona eburnea. The conditioned stimuli (CS) were mango (M) and guava (G); the unconditioned stimuli (US) were unscented-US (U) and scented-US (S). Experience with previous scent–reward associations were recorded as: same (when a single odor was used each day) and different (when two scents were used, a different one each day). Experiment code letters refer to scent used day 1, scent used day 2, and type of reward: mango mango unscented (MMU), guava guava unscented (GGU), mango mango scented (MMS), guava guava scented (GGS), mango guava unscented (MGU), guava mango unscented (GMU), guava mango scented (GMS), and mango guava scented (MGS).

Experiment Number	Experiment Code	Bee Sample (n Size)		Conditioned Stimulus (CS) SCENT		Unconditioned Stimulus (US) REWARD		Previous Scent–Reward Experience
		Day 1	Day 2	Day 1	Day 2	Day 1	Day 2
1	MMU	9	9	M	M	U	U	Same
2	GGU	11	9	G	G	U	U	Same
3	MMS	9	9	M	M	S	S	Same
4	GGS	10	4	G	G	S	S	Same
5	MGU	10	8	M	G	U	U	Different
6	GMU	11	11	G	M	U	U	Different
7	GMS	12	11	G	M	S	S	Different
8	MGS	11	11	M	G	S	S	Different