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. 2019 Oct 26;11(11):553. doi: 10.3390/pharmaceutics11110553

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Recellularization of bone marrow dendritic cells (BMDCs) into decellularized lymph node (dLN) scaffolds. (A) Immunofluorescence staining with CD11c⁺ BMDC in the dLN scaffolds. BMDCs were cultured inside dLN for 24 h with 100 or 10 µg/mL OVA antigen, and 100 µg/mL CPG-ODN stimulation. Flow cytometry was used to measure the expressions of (B) CD80, (C) CD86, and (D) major histocompatibility complex-ІІ (MHC-ІІ). (E) The geometric mean fluorescence intensities (gMFIs) of CD80, CD86, and MHC-ІІ. (Data are presented as the mean ± SD, *: p < 0.01.).