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Cytokine production from splenocytes after ex vivo stimulation. Splenocytes from mice receiving different levels of immunization were obtained and cultured with 100 µg/mL OVA antigen for 3 d. The supernatants were collected, and ELISA was used to determine the production of cytokines (A) interferon gamma (IFN-γ), (B) interleukin (IL)-4, (C) IL-6, and (D) IL-2. (Data are presented as the mean ± SD, *: p < 0.01, #: p < 0.001.).
