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. 2019 Nov 4;9(11):696. doi: 10.3390/biom9110696

Figure 8.

Figure 8

Increased phosphorylation of p38 mitogen-activated protein kinase (MAPK) by TET treatment in HCoV-infected MRC-5 cells with no antiviral synergistic effect of TET and anisomycin. (A) Cells were infected with HCoV-OC43, and 5 μM TET (vehicle 0.025% DMSO) was added to the cell cultures. Cell lysates were harvested at 30, 60, and 90 min post-infection. Western blot analysis was performed to determine the extent of p38 phosphorylation (Thr180/Tyr182). Total p38 was used as a control. (B) MRC-5 cells treated with 25, 50, or 100 nM ANM were infected with the virus in the presence or absence of 0.3 μM TET. Cell viability was assessed using the MTS assay at four dpi. Data are presented as mean ± SEM of three independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001 versus the vehicle group (one-way ANOVA plus Tukey’s multiple comparisons test).