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. 2019 Nov 13;15:248–260. doi: 10.1016/j.omto.2019.10.008

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© 2019.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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miR-424-5p Promotes Anoikis Resistance in Thyroid Cancer Cells

(A) The effects of miR-424-5p on apoptotic ratio in the indicated thyroid cancer cells via annexin V-FITC/PI staining. Error bars represent the mean ± SD of three independent experiments. (B) The effects of miR-424-5p on mitochondrial potential in the indicated thyroid cancer cells via JC-1 staining assay. Error bars represent the mean ± SD of three independent experiments. (C and D) Analyses of the activities of caspase-3 (C) and caspase-9 (D) were detected by the cleaved forms of these two proteins. Error bars represent the mean ± SD of three independent experiments. (E) Western blotting analysis of BAX, BAD, BCL2L1, and BCL2 expression in the indicated thyroid cancer cells. α-Tubulin served as the loading control. (F and G) The effects of miR-424-5p on migration (F) and invasion (G) ability in the indicated thyroid cancer cells. Error bars represent the mean ± SD of three independent experiments. *p < 0.05.