Figure 5.

miR-424-5p Inhibits Activity of the Hippo Signaling Pathway

(A) Activity of luciferase reporter constructs of several signaling pathways was examined in the miR-424-5p-overexpressing or -silencing thyroid cancer cells. (B) TEAD transcriptional activity was assessed by HOP-Flash luciferase reporter in the indicated cells. Error bars represent the mean ± SD of three independent experiments. (C) Representative immunofluorescent images of the indicated thyroid cancer cells immunostained with YAP antibody (red), phalloidin (green), or DAPI (blue). Each bar represents the mean values ± SD of three independent experiments. (D and E) Real-time PCR analysis of CTGF, CYR61, SOX9, HOXA1, RPL13A, and PPIA in the B-CPAP (D) and K1 (E) cells. Error bars represent the mean ± SD of three independent experiments. *p < 0.05.