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. 2019 Nov 13;15:248–260. doi: 10.1016/j.omto.2019.10.008

Figure 6.

Figure 6

miR-424-5p Targets WWC1, SAV1, and LATS2

(A) Predicted miR-424-5p targeting sequence and mutant sequences in 3′ UTRs of WWC1, SAV1, and LATS2. (B) Western blot analysis of WWC1, SAV1, LATS2, p-MST1/2, MST1, p-LATS1, LATS1, p-YAP, YAP, and TAZ expression in the indicated cells. α-Tubulin and p84 were used as total and nuclear loading controls, respectively. (C and D) microribonucleoprotein (miRNP) IP assay showing the association between miR-424-5p, and WWC1, SAV1, and LATS2 transcripts in B-CPAP (C) and K1 (D) cells. Pulldown of IgG antibody served as the negative control. Each bar represents the mean values ± SD of three independent experiments.(E and F) Luciferase assay of cells transfected with pmirGLO-3′ UTR reporter of WWC1, SAV1, and LATS2 in the miR-424-5p-overexpressing and -silencing B-CPAP (E) and K1 (F) cells. Each bar represents the mean values ± SD of three independent experiments. *p < 0.05.