Fig. 2.

(A) RTCA-based cell viability dose response curves for the 28-flavor mixture (red dots) and the corresponding base solution (blue dots). Green line corresponds to 50% of cell viability and dotted lines indicate extrapolated EC₅₀ values. (B, C) Tox-Scores (y-axis) are shown as a function of their corresponding p-values computed for (B) each individual flavoring substance present in the 28-flavor mixture and (C) the flavor mixtures without citronellol, alpha-pinene, or both after a 24-hour exposure. Red dot in b and c indicate flavoring substances and mixtures selected for subsequent HCS investigation. The vertical line indicates a p-value of 0.05. Each dot corresponds to one flavor solution, with those selected for subsequent HCS-based investigation shown by white dots. Abbreviation: SEM, standard error of the mean.