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. 2019 Nov 21;50:122–134. doi: 10.1016/j.ebiom.2019.10.060

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© 2019 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig 5 — GEFT influences the expression of EMT markers in RMS cells via the Rac1/Cdc42-PAK1 pathways. (a) Immunofluorescence assay confirmed that GEFT enhanced the fluorescence intensity of N-cadherin protein in RMS cells and reduced the fluorescence intensity of E-cadherin protein in RMS cells. (b) GEFT overexpression upregulated the expression levels of EMT related markers (N-cadherin, Snail, Slug, Twist, Zeb1, and Zeb2) and reduced the expression level of E-cadherin; the expression levels of EMT-related proteins were reduced by shGEFT, which also upregulated the expression level of E-cadherin. (c and d) The expression level of E-cadherin increased, whereas the expression levels of N-cadherin and Snail, Slug, Twist, ZEB1, and ZEB2 were reduced after NSC23766 or ZCL278 interference. (e) GEFT can promote the expression of PAK1, and NSC23766 or ZCL278 can inhibit the expression of PAK1. (f) The expression level of E-cadherin increased and the expression levels of N-cadherin and EMT-inducing transcription factors were reduced after IPA-3 interference.