Increased levels of PCAF in human OA cartilage and TNF-α-treated human chondrocytes. (A, B) Representative H&E staining and immunofluorescence staining of PCAF in cartilage tissues from normal and OA patients. (scale bar: 200 µm [A], 100 µm [B]). (C, D) PCAF and H3K9ac levels in chondrocytes derived from OA patients and controls. (E, F) PCAF and H3K9ac levels in human chondrocytes stimulated with 20 ng/ml TNF-α for 24 h. All results are presented as the means ± SDs of six duplicate experiments. *P < 0.05, **P < 0.01.