Fig. 2.

Over-expression of LSAMP-AS1 inhibits EMT, proliferation, migration and invasion of PCa cells. A, The quantitation of LSAMP-AS1 expression in human PCa cell lines and the normal prostate epithelial cells. B, The immunofluorescence staining to detect the distribution and expression of EMT-related proteins (400 ×). C-D, The protein bands and levels of EMT-related proteins normalized to β-actin measured by Western blot analysis. E, The cell proliferation assessed by MTT assay. F-G, The protein bands and levels of proliferation-related proteins normalized to β-actin measured by Western blot analysis. H-I, The cell migration and corresponding quantitation measured by Transwell assay (200 ×). J-K, The cell invasion and corresponding quantitation measured by Transwell assay (200 ×). l-M, The protein bands and levels of invasion and migration-related proteins normalized to β-actin measured by Western blot analysis. The data were measurement data and presented by mean ± standard deviation. Data in Panel E were analyzed by repeated measures ANOVA, followed by Bonferroni post hoc test. Data in Panel A, D, G, H, I, K, and M were analyzed by one-way ANOVA, followed by Tukey's post hoc test. Cell experiment was independently conducted 3 times. * p < 0.05 versus the RWPE-1 cell line or the empty vector group. # p < 0.05 versus the sh-NC group.