Fig. 5.

miR-183–5p targets and negatively regulates DCN gene. A, The genes in the intersection of down-regulated genes in the miRWalk database and GSE38241 microarray data. B, The core gene DCN was screened out by the String database. C, microRNA.org (http://www.microrna.org/) was used to predict the binding sites between miR-183–5p and DCN. D, The relative expression of DCN in PCa tissues and BPH tissues determined by RT-qPCR. E, Kaplan-Meier analysis for OS and DFS of PCa patients with high and low expression of DCN. F, Pearson correlation analysis of miR-183–5p expression and DCN expression in PCa tissues. G, The dual-luciferase reporter gene assay was applied to verify the binding of miR-183–5p to DCN. H, RNA-pull down assay was performed to confirm the binding of miR-183–5p to DCN. I-K, RIP assay was conducted to detect the binding of miR-183–5p to LSAMP-AS1 and DCN (* p < 0.05 versus the IgG group in Panel I, * p < 0.05 versus the empty vector group in Panel J and * p < 0.05 versus the sh-NC group in Panel K). * p < 0.05 versus the miR-183–5p-bio-mut group. Data in Panel D were analyzed by independent-sample t-test. Data in Panel G and H were analyzed by one-way ANOVA, followed by Bonferroni post hoc test. The cell experiment was repeated 3 times independently. The data were measurement data and presented by mean ± standard deviation. * p < 0.05 versus the BPH group or the mimic-NC group.