Fig. 6.

Over-expression of LSAMP-AS1 up-regulates DCN gene by sponging miR-183–5p to inhibit PCa cell EMT, proliferation, migration and invasion. A, Immunofluorescence staining to detect the distribution and expression of EMT-related proteins (400 ×). B-C, The protein bands and levels of EMT-related proteins normalized to β-actin measured by Western blot analysis. D, The cell proliferation assessed by MTT assay. E-F, The protein bands and levels of proliferation-related proteins normalized to β-actin measured by Western blot analysis. G-H, The cell migration and corresponding quantitation measured by Transwell assay (200 ×). I-J, The cell invasion and corresponding quantitation measured by Transwell assay (200 ×). K-L, The protein bands and levels of invasion and migration-related proteins normalized to β-actin measured by Western blot analysis. Data in Panel D were analyzed by repeated measures ANOVA, followed by Bonferroni post hoc test. Data in Panel C, F, H, J, and L were analyzed by one-way ANOVA, followed by Tukey's post hoc test. The data were measurement data and presented by mean ± standard deviation. * p < 0.05 versus the LSAMP-AS1 + mimic-NC group. # p < 0.05 versus the LSAMP-AS1 + sh-NC group. & p < 0.05 versus the LSAMP-AS1 + miR-183–5p mimic.