Figure 4.
Active immunizations with Aβ3–10-KLH vaccine reduced protein expression of p-tau (AT8 and AT180).
(A, B) Immunoblotting results of AT8 (phosphorylation sites Ser202 and Thr205) and AT180 (phosphorylation site Thr231). Protein expression levels of AT8 and AT180 were significantly lower in the Aβ3–10-KLH group compared with the PBS group (*P < 0.05). Data are expressed as mean ± SD (n = 6), and analyzed by one-way analysis of variance followed by the least significant difference test. All experiments were conducted at least in triplicate. 3×Tg-AD: Amyloid precursor protein/presenilin-1/tau transgenic mouse models; Aβ: amyloid-beta; KLH: keyhole limpet hemocyanin; PBS: phosphate-buffered saline; WT: wild-type.