Figure 3.

Effects of other types of cell death inhibitor including ZVAD-fmk (A), 3-MA (B), Nec-1 (C) and DFO (D) on the cell viability of HT-22 cells treated with Glu as detected by MTT.

Cell viability are expressed the optical density ratio to control group. Data are expressed as the mean ± SD. ##P < 0.01, vs. control group; *P < 0.05, **P < 0.01, vs. Glu group (one-way analysis of variance followed by Tukey’s post-hoc test). Quantification data represented the mean of three experiments in duplicates. 3-MA: 3-Methyladenine; DFO: deferoxamine mesylate salt; Glu: glutamate; HT-22 cell: a mouse hippocampal neurons cell; MTT: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; Nec-1: necrostatin-1; ZVAD-fmk: Z-Val-Ala-Asp (OMe)-fluoromethyl ketone.