Figure 6.

Western blot analyses of the differentially expressed growth factors in the distal nerve stump following sciatic nerve transection.

(A) Protein bands of HGF, CNTF and GDNF were measured by Western blot analysis. β-Actin was used as the reference protein. (B) Quantitative result of HGF, CNTF and GDNF expression. (C) The fold changes of HGF, CNTF and GFR alpha-1 were measured by protein microarray. Data represent the mean ± SEM (n = 4) from three independent experiments, statistically analyzed by one-way analysis of variance followed by Tukey’s post hoc test. *P < 0.05, **P < 0.01, vs. control group (ctrl; 0 day after nerve injury). CNTF: Ciliary neurotrophic factor; GDNF: glial cell line-derived neurotrophic factor; HGF: hepatocyte growth factor.