Fig. 7.

The ethanol-induced cytoplasmic mislocalization of RUNX3 further promotes the aggressiveness of CRC cells via EMT by targeting Snail. (A) Immunofluorescence assay of RUNX3 protein in cells transfected with the NLS-RUNX3 vector. (B) A Transwell assay with Matrigel was used to investigate the invasive ability of CRC cells treated with ethanol and/or NLS-RUNX3. (C) A wound-healing assay was used to investigate the motility of CRC cells treated with ethanol and/or NLS-RUNX3. (D) Representative western blots for TGF-β signalling-associated proteins. The values under the membrane represent the expression of genes normalized to the expression of the reference gene GAPDH or H3K4. “N-RUNX3”, “E-ca”, “βcat”, “FN”, “N-ca”, and “Vim” indicate nuclear-RUNX3, E-cadherin, β-catenin, fibronectin, N-cadherin and vimentin, respectively. *P < 0.05 vs the control; ^$P < 0.05 vs 200 EtOH.