Fig. 5.

Sera collected during post-transplant period show superior neutralization capacities of vaccinated seronegative transplant patients in comparison to placebo.

Strain Merlin was incubated with sera from seronegative patients, or pooled patient seropositive or seronegative sera as controls (n = 6) and used to inoculate HFFs in vitro (MOI=1). Infection was measured by IE staining and the proportion of infected cells calculated by counterstaining nuclei with DAPI. A) The average percentage infection of all the vaccinated and non-vaccinated heat inactivated patient sera; vaccine recipients are shown in blue (lower line) and those who received placebo are shown in black (upper line) B) The average percentage infection of all the vaccinated and non-vaccinated heat inactivated patient sera; patients who developed viraemia post-transplant are shown in red (upper line) and those who did not are depicted in black (lower line). Serum samples were collected at the time of transplantation and on days: 7, 35, 63 and 90 post-transplant. A comparison of neutralisation at each timepoint was performed by Mann U Whitney Test. *p<0.05, **p<0.01 and ns=non-significant difference.