Fig. 6.

The neutralising activity in the seronegative vaccine sera is inhibited following pre-absorption with recombinant gB.

Sera from healthy seronegative (sero-ve) and seropositive (sero+ve) individuals and sera from vaccinated seronegative SOT recipients collected 35 days post-transplant were diluted 1/10 and incubated with the virus prior to infection of the fibroblasts for 1 h (black bars). Alternatively, virus was incubated with ITC88 (100ug/ml) for 1 h prior to infection (ITC88 black bar). Additionally, each serum and ITC88 were also pre-incubated with the vaccine gB antigen prior to incubation with the virus in parallel (grey bars). Infection was measured by IE staining and the proportion of infected cells calculated by counterstaining nuclei with DAPI.