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. 2019 Nov 19;50:23–33. doi: 10.1016/j.ebiom.2019.11.014

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© 2019 The Author(s)

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig 4 — ANXA2 is a receptor for YadC.

(a) Analysis of 5637 cell membrane proteins binding to HA-YadC by far western blotting and LC-MS/MS. The peptides detected and peptide coverage percentage of ANXA2 are shown. Detected peptides belonging to ANXA2 are indicated in red. (b) Co-immunoprecipitation analysis of the interaction between FLAG-YadC with ANXA2 in 5637 cells. α, anti-. (c) Co-immunoprecipitation analysis of the interaction between the purified ANXA2 and FLAG-YadC protein. (d) Immunofluorescence analysis of ANXA2 and HA-YadC localization in 5637 cells. Blue, nucleus; Green, ANXA2; Red, HA. Scale bar, 20 μm. (e) Immunofluorescence analysis of ANXA2 and E. coli localization in 5637 cells. Blue, nucleus; Red, LPS; Green, ANXA2. Scale bar, 8 μm. (f) Immunofluorescence analysis of ANXA2 and E. coli localization in mouse bladder tissue infected with CFT073. Blue, nucleus; Green, E. coli; Red, ANXA2. Scale bar, 500 μm and 50 μm.