Table 5.

Overview of accuracy and performance running scSplit on five multiplexed scRNA-seq datasets, with one CPU and 30 GB RAM

scSplit vs demuxlet	Mix 1	Mix 2	Mix 3	Mix 4	Mix 5
Mixed samples	7	8	8	8	7
Number of cells	914	8 137	5 165	6 977	7 428
Reads per cell	86 148	16 386	21 265	18 572	19 657
informative SNVs	15 848	26 830	26 162	23 224	41 993
Build matrices	10 min	23 min	18 min	21 min	35 min
Assign cells	4 min	47 min	23 min	45 min	50 min
Singlet TPR	0.94	0.93	0.94	0.93	0.93
Singlet FDR	0.06	0.07	0.06	0.07	0.07
Doublet TPR	0.52	0.17	0.15	0.17	0.08
Doublet FDR	0.48	0.83	0.85	0.83	0.92
Cohen’s Kappa	0.86	0.78	0.68	0.77	0.76

(TPR: True Positive Rate; FDR: False Discovery Rate)