Table 1.

Primer sequences

Gene	Primer/probe	Sequence
Pf18S	18S forward	5′GTAATTGGAATGATAGGAATTTACAAGGT 3′
Pf18S	18S reverse	5′TCAACTACGAACGTTTTAACTGCAAC 3′
Pf18S	18S probe	6FAM-AACAATTGGAGGGCAAG–MGBNFQ
Dhps	Outer forward	5′ GATTCTTTTTCAGATGGAGG 3′
Dhps	Outer reverse	5′ TTCCTCATGTAATTCATCTGA 3′
Dhps	Inner forward	5′ AACCTAAACGTGCTGTTCAA 3′
Dhps	Inner reverse	5′ AATTGTGTGATTTGTCCACAA 3′
Dhfr	Outer forward	5′ TGATGGAACAAGTCTGC 3′
Dhfr	Outer reverse	5′ ACTTTGTTTATTTCCATTCA 3′
Dhfr	Inner forward	5′ TGTGCATGTTGTAAGGTTGA 3′
Dhfr	Inner reverse	5′ GATACTCATTTTCATTTATTTCTGGA 3′
Dhps	540 K	5′ ATTAAACAACTCTTAACTACACAAGGAAATCCACATACAATGGATA 3′
Dhps	540 E	5′ CTTTCTTAATACATTACAACATACGAAATCCACATACAATGGATG 3′
Dhps	581 A	5′ CATAAATCTTCTCATTCTAACAAATTGATATTGGATTAGGATTTGC 3′
Dhps	581 G	5′ CAAACAAACATTCAAATATCAATCTTGATATTGGATTAGGATTTGG 3′
Dhfr	164 I	5′ CATAAATCTTCTCATTCTAACAAAGAAATTAAATTACTATAAATGTTTTATTA 3′
Dhfr	164 L	5′ CAAACAAACATTCAAATATCAATCGAAATTAAATTACTATAAATGTTTTATTT 3′

Listed are primer sequences used in qPCR to confirm P. falciparum infection and quantify parasite density (Pf18S), nested PCR for amplifying Dhps and Dhfr for ASPE reactions for hybridisation with different alleles. Sequence highlighted in italic is the Luminex tag sequence

qPCR quantitative polymerase chain reaction, Dhps dihydropteroate synthase; Dhfr dihydrofolate reductase, ASPE Allele Specific Primer Extension