Muffat et al. 2016 [21] |
Muffat-MGL |
MECP2−/−
|
smaller microglia diameter. |
Gosselin et al. 2017 [33] |
Muffat-MGL |
n/a |
The transcriptome of MGLs closely resembles primary microglia cultured in vitro but differ significantly from microglia ex vivo. |
Muffat et al. 2018 [34] |
Muffat-MGL |
n/a |
Infected primitive yolk sac macrophages may act as ZIKV reservoir during pregnancy and establish brain infection upon colonising the developing CNS. |
Lin et al. 2018 [35] |
Muffat-MGL |
APOE (ε3 vs. ε4) |
APOE4 perturbs several microglial functions preventing microglia from clearing Aβ from AD brain organoids effectively. |
Brownjohn et al. 2018 [36] |
Haenseler-MGL |
TREM2T66M; −W50C
|
loss of TREM2 surface expression; reduced mature isoforms; reduced TREM2 proteolysis. |
Garcia-Reitboeck et al. 2018 [37] |
Haenseler-MGL |
TREM2T66M; −W50C
|
reduced or absent soluble TREM2 in supernatants. |
Xiang et al. 2018 [13] |
Haenseler-MGL (modified) |
TREM2R47H
|
normal TREM2 mRNA and protein expression and splicing patterns. |
Piers et al. 2019 [38] |
Haenseler-MGL (Xiang) |
TREM2T66M;
−W50C;
−R47H
|
metabolic deficits: a reduced mitochondrial respiratory capacity and an inability to perform a glycolytic immunometabolic switch, due to dysregulated PPARγ/p38MAPK signalling. |
Hasselmann et al. 2019 [39] |
Abud-MGL (McQuade) |
TREM2R47H
|
HPSC-derived hematopoietic progenitors engraft in the postnatal mouse brain and adopt the transcriptional identity of human primary microglia. TREM2 mutant microglia displayed reduced migration towards Aβ plaques. |
Claes et al. 2019 [40] |
Douvaras-MGL (modified) |
TREM2R47H;
+/−;
−/−
|
Isogenic hetero- and homozygous TREM2 knockouts resulted in reduced phagocytosis of E. coli fragments in vitro and amyloid plaques in AD mouse brain slices. TREM2R47H mutant MGLs displayed normal phagocytosis. |
Mancuso et al. 2019 [41] |
Douvaras-MGL (modified) |
n/a |
HESC-derived monocytes engraft in the postnatal mouse brain and adopt the transcriptional identity of human primary microglia. |
Takata et al. 2017 [30] |
Takata-MGL |
MEFVM694V
|
increased secretion of pro-inflammatory cytokines; increased ASC-speck formation. |