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. 2019 Dec 18;10:396. doi: 10.1186/s13287-019-1483-7

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — Osteogenic differentiation on different substrates. a ALP, b RUNX2, c OCN, and d POSTN expression were analyzed on culture day 7 in osteogenic medium by real-time PCR. e RUNX2 and POSTN expression were analyzed on culture day 7 in osteogenic medium by western blot. f The relative density of western blot using NIH Image J. g After osteogenic induction on different substrates for 21 days, mineral deposits were detected by alizarin red staining. Scale bar = 200 μm. h Quantitation of alizarin red staining of day 21 cultures. All values represent the mean ± SD; *p < 0.05 and **p < 0.01