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. 2019 Dec 18;10:396. doi: 10.1186/s13287-019-1483-7

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 7 — Angiogenic and adipogenic differentiation on different substrates. a, b Angiogenesis-related genes. a VEGF and b CD31 expression analyzed on culture day 7 in angiogenic medium by real-time RT-PCR. c, d Adipogenesis-related genes. c LPL and d PPARγ2 expression levels were analyzed on culture day 7 in adipogenic medium by real-time RT-PCR. e After adipogenic induction on different substrates for 21 days, lipid-laden lobules were detected by Oil Red O staining. Scale bar = 20 μm. f Quantitation of Oil Red O staining of day 21 cultures after isopropanol extraction. All values represent the mean ± SD; *p < 0.05 and **p < 0.01