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. 2019 Dec 18;10:401. doi: 10.1186/s13287-019-1516-2

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Size distribution analysis of hBM-MSC-sEVs performed with NTA and TEM confirmed small EV category assignment. a hBM-MSC-sEVs from (i) donor 1, (ii) donor 2, (iii) donor 3, (iv) donor 4, and (v) donor 5 were characterized by nanoparticle tracking analysis (NTA) as a means to estimate the hBM-MSC-sEV concentration and determine the EV size distribution. (vi) Superimposed NTA results of all five donors. X-axis designates size (nm) and y-axis designates concentration (mean) at different sizes of five individual healthy hBM-MSC donors (n = 5 donors). b Live cell counts of hBM-MSCs ± S. D, total mean concentration of hBM-MSC-sEV ± S. D, number of hBM-MSC-sEVs produced per hBM-MSC as a method of EV characterization, mean and mode size ± SD of hBM-MSC-sEVs, D10, D50, and D90 are shown as a method of nanoparticle size analysis. c Transmission electron microscopy (TEM) analysis confirmed the presence of hBM-MSC-sEV with the expected morphology, bilayer membrane, and size of small EVs (< 200 nm) (scale bars: D1, D2, D4, D5 = 200 nm, D3 = 100 nm). The data shown in a and b represent five hBM-MSC donors (n = 5 donors) analyzed in three independent trials (n = 3 experiments)