Fig. 3.

Flow cytometric analysis of CD9, CD63, and CD81 tetraspanin expression of the CD63+ hBM-MSC-sEVs population. a Representative flow cytometric plots of CD63 immuno-precipitated hBM-MSC-sEV population (i), counterstained for CD63 (ii), CD81 (iii), and CD9 (iv) (blue) against isotype control IgG1κ (gray) which served as a negative control. b Quantification of the median fluorescent intensity (MFI) data of the five hBM-MSC-sEV samples (n = 5 hBM-MSC donors) analyzed in three independent trials (n = 3 experiments). c Representative flow cytometric dot plots of hBM-MSC-sEV labeled MACSplex beads following CD63 detection. (i) Representative plots of buffer only negative control containing no nanoparticles (i and ii). Representative plots of hBM-MSC-sEV samples detected with CD63 counterstaining (iii and iv). d The IgG Isotype subtracted MFI data representative of five hBM-MSC-sEV samples (n = 5 hBM-MSC donors) analyzed in two independent trials (n = 2 experiments) displaying the relative surface abundance of various human CD markers