Fig. 6.

Validation of NRP1 selected from the twofold enriched hBM-MSC-sEV dataset by Western blot analysis. a Mass spectrometry-based proteomics data of NRP1 identified from the hBM-MSC and hBM-MSC-sEV groups that allowed its identification and quantification by LC-MS/MS. b Western blot validation of NRP1 mass spectrometry data showing the enrichment of NRP1 in hBM-MSC-sEVs as compared to hBM-MSCs from the samples analyzed by MS. c Quantification of % NRP1 abundance levels from the hBM-MSC and hBM-MSC-sEV groups obtained from b results. d Verification by Western blot of NRP1 from three additional EV production batches from all five hBM-MSC-sEV samples. e Quantification of NRP1 relative fluorescent units from the hBM-MSC-sEV samples from all five hBM-MSC donors. The data represent five hBM-MSC donors (n = 5 donors; donors 1–5) analyzed in two independent WB experiments from batch #1 EV production (b, c) or four independent WB experiments (d, e) from three additional separate EV production batches (batches #2–4)