Fig. 3.

RA caused reduced levels of H3K27me3 at Hox loci in mouse NTDs embryos. a Cranial neural tissue of normal and RA-induced mouse NTDs was harvested at E10.5, and analyzed by western blotting. Numbers at the bottom were generated by quantification (ImageJ) of the H3K27me3/2/1 signal normalized to the H3 signal. b Immunohistochemistry staining was performed on transverse sections, for detecting H3K27me3 in NC and NTDs mouse embryos of E10.5. Scale bar: 50 μm. Areas of H3K27me3 positive cells were quantified by using ImageJ. Data are shown as the mean (SD; n = 5). *P < 0.05. c Comparison of average ChIP-Seq reads densities for H3K27me3 between NC and NTDs mouse embryos. d ChIP-Seq enrichment profiles for H3K27me3 levels in NC and NTDs mouse embryos. e ChIP-Seq density profiles for H3K27me3 at the mouse Hoxa–d clusters in NC and NTDs mouse embryos. f ChIP-Seq density profiles for H3K27me3 at the 10 Hox genes in NC and NTDs mouse embryos