Table 2.
Overview of antibodies and incubation times
| Antibody | Origin | Target | Company | Catalogue number | Dilution | Incubation |
|---|---|---|---|---|---|---|
| CD3 | Rabbit | T cells | Dako/Agilent | A0452 | 1:2000 | Overnight |
| CD8 | Mouse | T cells and NK | Dako/Agilent | M7103 | 1:100 | Overnight |
| CD68 | Mouse | CD68+ macrophages | DakoCytomation | M0814 | 1:400 | Overnight |
| CD206 (MMR) | Goat | CD 206+ macrophages | DakoCytomation | AF2534 | 1:400 | Overnight |
| CD244 | Goat | CD28null T cells | R&D Systems | AF1039 | 1:100 | Overnight |
| DAPI | DNA | Thermo Fisher | 62248 | 1:10000 | 10 s | |
| FoxP3 | Mouse | Treg cells | Thermo Fisher | 11-4777-82 | 1:500 | Overnight |
| Laminin | Rabbit | Basal membrane | DakoCytomation | Z0097 | 1:2000 | Overnight |
| MHC—fast | Mouse | Type 2 fibres | Sigma-Aldrich | M4276 | 1:1000 | 30 min |