Fig. 2.

knockdown of KIAA1429 impairs the tumor growth and metastasis in vivo. a and b Tumor volume and tumor weight in subcutaneous xenografts models with indicated SK-Hep1 and HCCLM3 cells. Scale bars = 5 mm. c and d Representative brightfield and fluorescence photographs of intrahepatic metastatic nodules in orthotopic implantation models with indicated SK-Hep1 and HCCLM3 cells. Scale bars = 5 mm. e and f Representative microscopic views of intrahepatic metastatic foci from indicated SK-Hep1 and HCCLM3 cells in tissue sections of livers using HE staining. Scale bars = 100 μm. g and h Representative bioluminescence photographs of the murine tail vein injection lung metastasis models at indicated times by imaging with the IVIS@ Lumina II system. i and j Representative brightfield and fluorescence photographs of pulmonary metastatic nodules in lung metastasis models with indicated SK-Hep1 and HCCLM3 cells. Scale bars = 2 mm. k and l Representative microscopic views of pulmonary metastatic foci from indicated SK-Hep1 and HCCLM3 cells in tissue sections of lungs using HE staining. Scale bars = 100 μm. Data are presented as mean ± SEM. **P < 0.01, ***P < 0.001