Fig. 4.

The GATA3 3′ UTR maintains the KIAA1429-mediated m6A regulation. a MeRIP-seq of the distribution of m6A peaks along GATA3 mRNA in KIAA1429-inhibited cells. b MeRIP-qPCR analysis of fragmented or intact GATA3 mRNA or GATA3 pre-mRNA in control and KIAA1429-depleted cells. c Western blot analysis of GATA3 expression in SK-Hep1 and HCCLM3 cells co-transfected with GATA3 CDS-3′ UTR or GATA3 CDS vector and KIAA1429 siRNAs or the control. d Relative luciferase activity in HEK293T cells co-transfected with luciferase reporter pmirGLO-GATA3-WT or pmirGLO-GATA3-MUT and KIAA1429 siRNAs or the control. Data are shown as the relative ratio of firefly luciferase activity to renilla luciferase activity. e Relative luciferase activity in HEK293T cells co-transfected with luciferase reporter pmirGLO-GATA3-WT or pmirGLO-GATA3-MUT and HuR siRNAs or the control. Data are shown as the relative ratio of firefly luciferase activity to renilla luciferase activity. f and g RIP-qPCR analysis of the enrichment of GATA3 pre-mRNA on HuR relative to IgG in control and KIAA1429-depleted cells. h Western blot analysis of HuR, GATA3 and KIAA1429 expressions in SK-Hep1 and HCCLM3 cells transfected with HuR siRNAs or the control. Data are presented as mean ± SEM. NS: not significant; *P < 0.05, **P < 0.01, ***P < 0.001