Fig. 5.

IGF2BP2 is a functional target of miR-141 in the miR-141-mediated suppression of pancreatic cancer cell proliferation. a and b The effect miR-141 transfection after IGF2BP2 overexpression on pancreatic cancer cell proliferation as determined by CCK8 assays. c and d Apoptosis analysis of IGF2BP2-overexpressing cells after miR-141 transfection by flow cytometry. e Cell cycle analysis of IGF2BP2-overexpressing cells after miR-141 transfection by flow cytometry. f Pathways associated with IGF2BP2 expression by KEGG enrichment analysis. g Expression of p-AKT by western blot analysis. h Expression of related regulators by western blot analysis. GAPDH served as a loading control. Data are presented as the mean ± SD of at least three independent measurements. *P < 0.05, **P < 0.01, and ***P < 0.001. P < 0.05 was considered statistically significant