Fig. 2.
Aggregation of dermal and epidermal cells enhances the expression of WNT pathway-related genes. a–l Dissociated dermal (Der), epidermal (Epi), or mixed dermal-epidermal (Epi-Der) cells at a 1:1 ratio were collected for total mRNA extraction (Dis-0 h) without aggregation or were placed into suspension for 24 h to form aggregates, after which the aggregates were harvested at 24 h (Agg-24 h) for total mRNA extraction. A total 2 × 105 cells were used for this experiment. Total mRNAs from all conditions were analyzed for the expression of genes as indicated by RT-PCR, and the relative expression level of each gene was normalized with the housekeeping gene 36B4. The experiment was repeated three times (n = 3). Significance between groups labeled with black lines was established by P values as indicated: *P < 0.05,**P < 0.01;***P < 0.005, ****P < 0.001