Fig. 5.

Activation of the WNT pathway is essential for the formation of type I aggregates. a Fetal scalp-derived dermal cells mixed with foreskin-derived epidermal cells at a 1:1 ratio were cultured in suspension and harvested at different time points as indicated for RT-PCR analysis of LEF1, WNT10b, and K17 expression. *P < 0.05, **P < 0.01, and ***P < 0.005 when compared with the group at 0 h. b Representative images of 48 h aggregates formed from fetal scalp-derived dermal cells mixed with foreskin-derived epidermal cells at a 1:1 ratio in suspension cultures in the presence of DMSO (control), WNT3a or IWP-2. Bar = 500 μm. Black arrows indicate type I aggregates. c Quantification of the percentage of type I aggregates formed in b, **P < 0.01, ***P < 0.005 when compared to the control group