Figure 4.

Effects of nonsense mutation of ald encoding aldehyde dehydrogenase in ethylene glycol-producing C. glutamicum strains. Growth and carbon source consumption in recombinant C. glutamicum for the production of ethylene glycol. Optical densities at 600 nm (OD₆₀₀; solid symbol; solid line) and glucose (open symbol; dashed line) concentrations, xylose (solid symbol; solid line) and ethylene glycol (open symbol; dashed line) concentrations, and glycolate and xylonate (open symbol; dashed line) concentrations in the medium were measured. C. glutamicum SL-1 pIEARKT (black square) and SL-1A pIEARKT (red circle) were cultivated in the CgXII medium with a mixture of xylose (2% wt/vol) and glucose (2% wt/vol). The data represent mean values of triplicate cultivations, and the error bars represent standard deviations. See Table 1 for the strains used.