| Strains |
|
E. coli DH5α |
F- (80d lacZ M15) (lacZYA-argF)
U169 hsdR17(r– m+) recA1 endA1 relA1 deoR96 |
(40) |
|
C. glutamicum WT |
ATCC 13032,
wild-type strain |
ATCC |
|
C. glutamicum SL-1 |
ATCC 13032, ΔxylB (in-frame
deletion) |
this study |
|
C. glutamicum SL-1R |
SL-1, iolR::P9Ter (nonsense mutation) |
this study |
|
C. glutamicum SL-1A |
SL-1, ald::C49Ter (nonsense mutation) |
this study |
| Plasmids |
| pK19mobSacB-xylB |
KmR; pK19mobsacB
derivative containing a crossover PCR product covering the upstream
and downstream regions of xylB
|
this study |
| pBbEB1c-RFP |
ColE1 (Ec), pBL1 (Cg), Cmr, Ptrc, BglBrick sites, rfp, CoryneBrick vector |
(36) |
| pBbEB1c |
ColE1 (Ec), pBL1 (Cg), Cmr, Ptrc, BglBrick sites, CoryneBrick empty vector |
(36) |
| pBbEB1c-XylA |
pBbEB1c-derived
vector carrying xylA of E. coli codon-optimized
for C. glutamicum
|
(41) |
| pUC57-dte(co), -fucK(co),-fucA(co),
-aldA(co), -yqhD(co), -fucO(co) |
pUC57 vectors containing
a single gene: dte originated from P. cichorii and fucK, fucA, aldA, yqhD, and fucO originated from E. coli. All genes
were codon-optimized (co) for C. glutamicum
|
this study |
| pIEKAD |
pBbEB1c vector carrying
codon-optimized xylA-dte-fucK-fucA-aldA for C. glutamicum, Cmr Symbols: I, isomerase
(xylA); E, epimerase (dte); K, kinase
(fucK); A, aldolase (fucA); D, dehydrogenase
(aldA) |
this study |
| pIEAKD |
pBbEB1c
vector carrying
codon-optimized xylA-dte-fucA-fucK-aldA for C. glutamicum, Cmr
|
this study |
| pIEAKDT |
pIEAKD-derived vector carrying iolT1 of C. glutamicum for C. glutamicum
|
this study |
| pIEAKR |
pBbEB1c vector carrying
codon-optimized xylA-dte-fuckA-fucK- yqhD for C. glutamicum, Cmr Symbols: I, isomerase
(xylA); E, epimerase (dte); K, kinase
(fucK); A, aldolase (fucA); R, reductase
(yqhD) |
this study |
| pIEKAR |
pBbEB1c
vector carrying
codon-optimized xylA-dte-fucK-fucA-yqhD for C. glutamicum, Cmr
|
this study |
| pIEARK |
pBbEB1c vector carrying
codon-optimized xylA-dte-fucA-yqhD-fucK for C. glutamicum, Cmr
|
this study |
| pIEARKT |
pIEARK-derived vector carrying iolT1 of C. glutamicum for C. glutamicum
|
this study |
| pIEKAO |
pBbEB1c vector carrying
codon-optimized xylA-dte-fucK-fucA-fucO for C. glutamicum, Cmr Symbols: I, isomerase
(xylA); E, epimerase (dte); K, kinase
(fucK); A, aldolase (fucA); O, oxidoreductase
(fucO) |
this study |
| pIEAOK |
pBbEB1c
vector carrying
codon-optimized xylA-dte-fucA-fucO-fucK for C. glutamicum, Cmr
|
this study |
| pJYS1Ptac |
pBL1tsoriVC.g. Kmr pSC101 oriVE.c. PlacM-FnCpf1 Ptac-RecT lacIq
|
(39) |
| pJYS2_crtYf |
rep oriVC.g. Spr pMB1 oriVE.c. Pj23119-crRNA-crtYf
targeting the crtYf gene |
(39) |
| pcrRNA-iolR |
pJYS2 derivative, Pj23119-crRNA-iolR targeting iolR
|
this study |
| pcrRNA-ald |
pJYS2 derivative, Pj23119-crRNA-ald
targeting ald
|
this study |
