FIG 3.

In vitro interleukin 33 gene (il33) expression following stimulation by fecal bacteria isolated from ovalbumin (OVA)-induced allergic mice. Cells from colon-26, a mouse colon epithelial cell line, were stimulated by four species of viable bacteria isolated from the feces of OVA-allergic mice. il33 expression levels in each sample were normalized to those of a housekeeping gene (gapdh) and expressed as fold changes relative to levels in a nonstimulated sample. Results are presented as the mean ± SD (n = 3) and represent three independent experiments. *, P < 0.05.