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. 2019 Dec 17;88(1):e00656-19. doi: 10.1128/IAI.00656-19

FIG 5.

FIG 5

CC042 mice have altered bone marrow-resident hematopoietic progenitor populations. Flow cytometry analysis of hematopoietic progenitors in femoral bone marrow from C57BL/6J and CC042 mice. (A) Schematic diagram of the stages of hematopoietic stem cell differentiation. (B) Total number of bone marrow cells per femur averaged over the total number of cells collected for two femurs. (C) Gating scheme used to analyze Lin− Sca1+ cKit+ (LSK) cells. (D) Total LSK cells per femur by developmental stage. LSK cells progress through the HSC, MPP1, MPP2, MPP3, and MPP4 subsets. (E) Gating scheme used to analyze Lin− cKit+ Sca1− (LKS−) and common lymphoid progenitors (CLP). FSC-A, forward scatter area. (F) Total number of LKS− cells per femur grouped by progenitor stage. LKS− cells comprise common myeloid progenitors (CMP), granulocyte-macrophage progenitors (GMP), and megakaryocyte-erythroid progenitors (MEP). (G) Total number of CLPs per femur. The data in the graphs represent the mean ± SEM. Data are representative of those from three independent experiments. The cell populations are defined in Table 2. Significance was determined by Welch’s t test (B and G) and multiple t tests using the Holm-Sidak method (D and F). *, P < 0.05; **, P < 0.01.