FIG 11.

Egr-1-deficient BMMs display upregulated iNOS protein expression during P. aeruginosa infection. Wild-type (+/+) and Egr-1-deficient (−/−) BMMs were infected with P. aeruginosa 8821 at an MOI of 10 for 1 h, 2 h, 4 h, or 6 h or were left untreated (NT). Cell lysates were subjected to Western blotting for determining the protein levels of iNOS and actin as a loading control. Blots are representative of three independent experiments (A). Densitometry analysis of iNOS expression levels was normalized to actin (B) (n = 3 ± SEM; *, P < 0.05).